Variant-specific RT-qPCR for rapid screening of B.1.617 mutations in SARS-CoV-2Article Published on 2022-12-012022-11-15 Journal: The Libyan journal of medicine [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] B.1.617 B.1.617 lineage B.1.617.1 B.1.617.2 collected country Delta delta variant detect Diagnostic method evaluate kappa variant. L452R Libya Lineage multiplex RT-qPCR Mutation mutations new SARS-CoV-2 P681 PCR assay positive sample required RT- qPCR RT-qPCR RT-qPCR assay SARS-CoV-2 screened Sequencing Spread tested variant virus whole genome [DOI] 10.1080/19932820.2022.2121252 PMC 바로가기
Evolving strategy for an evolving virus: Development of real-time PCR assays for detecting all SARS-CoV-2 variants of concern진화하는 바이러스에 대한 진화하는 전략: 우려되는 모든 SARS-CoV-2 변이체를 검출하기 위한 실시간 PCR 분석법 개발Article Published on 2022-09-012022-09-11 Journal: Journal of virological methods [Category] COVID19(2023년), MERS, SARS, 변종, 진단, 치료기술, 치료제, [키워드] Alpha Amino acid amino acid change amino acid changes approach B.1.1.7 Beta Canada characterized clinical sensitivity clinical specificity complementary COVID-19 Critical cross-reactivity Delta detect development discriminatory mutation E gene E484K FIVE Gamma Genome sequencing implementation K417N L452R less limit of detection Lineage molecular MONITOR N501Y Negative predictive value nucleotide omicron Omicron variants P681 Pathogens PCR PCR assays positive Positive predictive value positive predictive values positive sample predictive values reaction real-time PCR assay real-time reverse transcriptase real-time RT-PCR replacement respiratory respiratory pathogen responsible reverse transcriptase rRT-PCR SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genome sequencing SARS-CoV-2 variant SARS-CoV-2 variants sensitivity Single nucleotide polymorphism single nucleotide polymorphisms specificity Spike protein subset Surveillance target the SARS-CoV-2 Variability variant variants variants of concern variants of concern. VoC VOCs while wild-type Δ69-70 [DOI] 10.1016/j.jviromet.2022.114553 PMC 바로가기 [Article Type] Article
Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant델타 스파이크 P681R 돌연변이는 알파 변종보다 SARS-CoV-2 적합성을 향상시킵니다.Article Published on 2022-05-172022-09-11 Journal: Cell Reports [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 Alpha variant Amino acid cleavage coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 CP: Microbiology Delta delta variant ENhance epithelial cell epithelial cells fitness full-length furin furin cleavage site human airway human airway culture human lung human lung epithelial cells IMPROVE Microbiology Mutation P681 P681H P681R pandemic primary human airway reduce reduced Replication S1 and S2 S1/S2 S1/S2 cleavage SARS-CoV-2 severe acute respiratory syndrome Coronavirus significantly spike mutation spike P681R subunits tissues variant viral entry viral entry. virus entry wild-type [DOI] 10.1016/j.celrep.2022.110829 PMC 바로가기 [Article Type] Article
Furin cleavage of the SARS-CoV-2 spike is modulated by O -glycosylationSARS-COV-2 스파이크의 푸린 절단은 O- 글리코 실화에 의해 조절된다.407 Published on 2021-11-032022-08-13 Journal: Proceedings of the National Academy of Sciences of [Category] Biochemistry, COVID-19, SARS, [키워드] O- abrogate Alpha alpha and delta variants Amino acid amino acids cells circumvent cleavage coronavirus decrease delta variant Delta variants dependent on expressed family members furin furin cleavage furin cleavage of S furin cleavage site GALNT GALNT enzyme global pandemic glycosylating S glycosylation highlight host O -glycosylation host-mediated O -glycosylation human respiratory cell human respiratory cells increase insertion insertion site modulated modulating Mutation O -glycosylation P681 P681 mutation P681 mutations position position 681 proline pseudoviral infectivity respiratory responsible resulting SARS-CoV-2 coronavirus SARS-COV-2 infection shown Significance spike Spike protein syncytia formation target targets the SARS-CoV-2 the spike protein tropism unique Viral viral infectivity viral infectivity/tropism [DOI] 10.1073/pnas.2109905118 PMC 바로가기 [Article Type] 407
Development of a Multiplex Tandem PCR (MT-PCR) Assay for the Detection of Emerging SARS-CoV-2 Variants신흥 SARS-COV-2 변이체의 검출을위한 멀티 플렉스 탠덤 PCR (MT-PCR) 분석의 개발Communication Published on 2021-10-082022-09-01 Journal: Viruses [Category] MERS, SARS, 변종, 진단, 치료기술, [키워드] absence Alpha Applying approach B.1.1.7 B.1.1.7 lineage Chain Reaction clinical isolates clinical setting Concordance defined detection development evaluated example facilitated FIVE Genome sequencing genomic hospital identify in vitro diagnostic test information international public health isolate K417 Lineage Mutation National nucleic acid nucleotide P681 PCR polymerase chain polymerase chain reaction polymorphism Polymorphisms presence or absence Public health responses reference strain S gene SARS-CoV-2 SARS-CoV-2 lineage Sequence analysis Sequencing single nucleotide Single nucleotide polymorphism SNP tandem turnaround time UK hospital utilised variant variants variants of SARS-CoV-2 [DOI] 10.3390/v13102028 PMC 바로가기 [Article Type] Communication
Variant Prediction by Analyzing RdRp/S Gene Double or Low Amplification Pattern in Allplex SARS-CoV-2 AssayArticle Published on 2021-10-082022-10-31 Journal: Diagnostics [Category] COVID-19, [키워드] abnormality acute respiratory syndrome Allplex SARS-CoV-2 Assay amplification amplification curve analyzed B.1.671.2 coronavirus delta variant diagnosing diagnostic genomic sequencing identified variant P681 pattern PCR amplification positive sample prediction RdRP RdRp gene Real-time PCR required RNA-dependent RNA polymerase RT-PCR test SARS-CoV-2 SARS-COV-2 infection sensitivity shown specificity spike gene Spread strain the SARS-CoV-2 virus variant variants viral mutation [DOI] 10.3390/diagnostics11101854 PMC 바로가기 [Article Type] Article